ppharmПедиатрическая фармакологияPediatric pharmacology1727-57762500-3089Издательство «ПедиатрЪ»10.15690/pf.v22i3.2915ppharm-2642Research ArticleОРИГИНАЛЬНАЯ СТАТЬЯORIGINAL ARTICLESСостав кишечной микробиоты у детей раннего возраста с IgE-опосредованной и не-IgE-опосредованной пищевой аллергией: одномоментное исследованиеThe Composition of the Intestinal Microbiota in Young Children with IgE-mediated and Non-IgE-mediated Food Allergies: Cross Sectional Studyhttps://orcid.org/0000-0001-6395-028XШукенбаеваР. А.ShukenbaevaRegina A.

Шукенбаева Регина Айратовна - аспирант кафедры факультетской педиатрии Института материнства и детства Российского национального исследовательского медицинского университета им. Н.И. Пирогова (Пироговский Университет)

119333, Москва, ул. Фотиевой, д. 10, тел.: +7 (926) 996-87 61

MD.

10, Fotievoy Str., Moscow, 119333

Shukenbaevar@mail.ruhttps://orcid.org/0000-0002-8717-2539БеляеваИ. А.BelyaevaIrina A.

Беляева Ирина Анатольевна - д.м.н., профессор РАН.

Москва

MD, PhD, Professor of the RAS.

Moscow

irinaneo@mail.ruhttps://orcid.org/0000-0002-4955-0121ТуртиТ. В.TurtiTatyana V.

Турти Татьяна Владимировна - д.м.н., профессор.

Москва

MD, PhD, Professor.

Moscow

turtit@mail.ruhttps://orcid.org/0000-0002-6677-2914БомбардироваЕ. П.BombardirovaElena P.

Бомбардирова Елена Петровна - д.м.н., профессор.

Москва

MD, PhD, Professor.

Moscow

babalena92@mail.ruРоссийский национальный исследовательский медицинский университет им. Н.И. Пирогова (Пироговский Университет)РоссияPirogov Russian National Research Medical UniversityRussian FederationРоссийский национальный исследовательский медицинский университет им. Н.И. Пирогова (Пироговский Университет); НИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»; Морозовская детская городская клиническая больницаРоссияPirogov Russian National Research Medical University; Pediatrics and Child Health Research Institute in Petrovsky National Research Centre of Surgery; Morozov Children’s City Clinical HospitalRussian FederationРоссийский национальный исследовательский медицинский университет им. Н.И. Пирогова (Пироговский Университет); НИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»; Научно-исследовательский институт организации здравоохранения и медицинского менеджментаРоссияPirogov Russian National Research Medical University; Pediatrics and Child Health Research Institute in Petrovsky National Research Centre of Surgery; Research Institute for Healthcare Organization and Medical ManagementRussian FederationНИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»РоссияPediatrics and Child Health Research Institute in Petrovsky National Research Centre of SurgeryRussian Federation202521072025223285293Copyright © Шукенбаева Р.А., Беляева И.А., Турти Т.В., Бомбардирова Е.П., 20252025Шукенбаева Р.А., Беляева И.А., Турти Т.В., Бомбардирова Е.П.Shukenbaeva R.A., Belyaeva I.A., Turti T.V., Bombardirova E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pedpharma.ru/jour/article/view/2642Обоснование

Обоснование. Изменения микробиоценоза в критические периоды онтогенеза приобретают особое значение, поскольку создают предпосылки для формирования отсроченной патологии. Однако у младенцев, страдающих аллергией, связи изменений кишечной микробиоты с определенными аллергенами изучены недостаточно.

Цель исследования — определить особенности состава кишечной микробиоты у детей раннего возраста с IgEопосредованной и не-IgE-опосредованной пищевой аллергией.

Методы

Методы. У младенцев 6–12 мес с проявлениями пищевой аллергии исследовали состав кишечной микробиоты (культуральный метод), определяли наличие IgE методом иммунохемилюминесцентного анализа, исследовали уровни сенсибилизации методом «Аллергочип ImmunoCAP ISAC». Полученные данные подвергнуты корреляционному анализу.

Результаты

Результаты. Наблюдались 56 доношенных детей с симптомами аллергии, из которых 15 (27%) рождены путем кесарева сечения, 12 (21%) получали антибиотики в перинатальном периоде, у 30 (54%) было рано прекращено исключительное грудное вскармливание. При оценке кишечной микробиоты снижение уровней симбионтов отмечено у 32 (57%) пациентов. Выявлено повышенное содержание Klebsiella spp. — у 21 (38%), Clostridium spp. — у 5 (9%), Enterobacter spp. — у 5 (9%), Escherichia coli лактозонегативных — у 11 (20%), Citrobacter spp. — у 4 (7%), Escherichia coli гемолитических — у 7 (13%) детей. По результатам иммунологических исследований пациенты были разделены на 2 подгруппы: с IgE-опосредованной (n = 10) и не-IgE-опосредованной пищевой аллергией (n = 46). У пациентов с IgE-опосредованной аллергией выявлены достоверные положительные связи: sIgE к альфа-лактальбумину и казеину (Bos d4, Bos d8), лизоциму (Gal d4) с содержанием Escherichia coli гемолитической (R = 0,31; 0,35; 0,37); sIgE к казеину (Bos d8), лизоциму (Gal d4) с содержанием Clostridium spp. (R = 0,30; 0,32).

Заключение

Заключение. Взаимосвязи IgE-опосредованной сенсибилизации к пищевым аллергенам и состава кишечной микробиоты являются основанием для разработки методов индивидуализированной коррекции аллергического фенотипа.

Background

Background. Changes in microbiocenosis during critical periods of ontogenesis are of particular importance, as they create prerequisites for the formation of delayed pathology. However, in infants with allergies, the association of changes in the intestinal microbiota with certain allergens has not been sufficiently studied.

The aim of the study is the determination the composition features of the intestinal microbiota in young children with IgE-mediated and non-IgE-mediated food allergies.

Methods

Methods. In infants 6–12 months old with food allergy symptoms, the composition of the intestinal microbiota was studied (culture method), the presence of IgE was determined by chemiluminescence immunoassay, and the levels of sensitization were studied by the ImmunoCAP ISAC method. The data obtained has been subjected to correlation analysis.

Results

Results. There were 56 full-term infants with allergy symptoms, of whom 15 (27%) were born by caesarean section, 12 (21%) received antibiotics in the perinatal period, and 30 (54%) stopped exclusive breastfeeding early. When assessing the intestinal microbiota, a decrease in symbiont levels was noted in 32 (57%) patients. An increased content of Klebsiella spp. was revealed — in 21 (38%), Clostridium spp. — 5 (9%) have Enterobacter spp. — in 5 (9%), Escherichia coli lactose-negative — in 11 (20%), Citrobacter spp. — in 4 (7%), Escherichia coli hemolytic — in 7 (13%). According to the results of immunological studies, patients were divided into 2 subgroups: with IgE-mediated (n = 10) and non-IgE-mediated food allergies (n = 46). In patients with IgE-mediated allergy, significant positive associations were found: SiGe to alpha-lactalbumin and casein (Bos d4, Bos d8), lysozyme (Gal d4) with hemolytic Escherichia coli content (R = 0.31; 0.35; 0.37); SiGe to casein (Bos d8), lysozyme (Gal d4) containing Clostridium spp. (R = 0.30; 0.32).

Conclusion

Conclusion. The interrelationships of IgE-mediated sensitization to food allergens and the composition of the intestinal microbiota are the basis for the development of methods for individualized correction of the allergic phenotype.

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The authors declare that there are no conflicts of interest present.