ppharmПедиатрическая фармакологияPediatric pharmacology1727-57762500-3089Издательство «ПедиатрЪ»10.15690/pf.v18i5.2294ppharm-2064Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзменение композиции кишечной микробиоты у детей с атопическим дерматитом 1–5 лет: одномоментное исследованиеChanges in Intestinal Microbiota Composition in 1–5 Years Old Children with Atopic Dermatitis: Cross Sectional Studyhttps://orcid.org/0000-0001-9813-6616ЮдинаЮ. В.YudinaJulia V.

Юдина Юлия Владимировна – аспирант кафедры педиатрии и детских инфекционных болезней Клинического института детского здоровья им. Н.Ф. Филатова, врач педиатр

123317, Москва, Шмитовский проезд, 29, elibrary SPiN: 7896-0260; телефон: +7 (499) 259-47-32

Julia V. Yudina

29 Shmitovsky Ave. 123317, Moscow, eLibrary SPIN: 7896-0260; +7 (499) 259-47-32 

yuliayudina1989@mail.ruhttps://orcid.org/0000-0002-1951-6424АминоваА. И.AminovaAlfiia I.

Аминова Альфия Иршадовна

119992, Москва, ул. Большая Пироговская, 19, eLibrary SPIN: 2529-8975; телефон: +7 (499) 248-88-41

Alfia I. Aminova

19 Bolshaya Pirogovskaya, Str., 119992, Moscow, eLibrary SPIN: 2529-8975; +7 (499) 248-88-41

aminova62@yandex.ruПродеусА. П.ProdeusAndrey P.

Продеус Андрей Петрович

123317, Москва, Шмитовский проезд, 29, AuthorID: 553740; телефон: + 7 (499) 259-13-07

Andrey P. Prodeus

29 Shmitovsky Ave. 123317, Moscow, AuthorID: 553740; + 7 (499) 259-13-07

prodeus@mail.ruhttps://orcid.org/0000-0002-9087-1656КорсунскийА. А.KorsunskiyAnatoly A.

Корсунский Анатолий Александрович

123317, Москва, Шмитовский проезд, 29, AuthorID: 493678; телефон: +7 (499) 256-21-62

Anatoly A. Korsunskiy

29 Shmitovsky Ave. 123317, Moscow, AuthorID: 493678; +7 (499) 256-21-62

dgkb9@zdrav.mos.ruПервый Московский государственный медицинский университет им. И.М. Сеченова (Сеченовский Университет); Детская городская клиническая больница № 9 им. Г.Н. Сперанского Департамента здравоохранения г. МосквыРоссияSechenov First Moscow State Medical University; G.N. Speransky Children’s Municipal Clinical Hospital № 9Russian FederationДетская городская клиническая больница № 9 им. Г.Н. Сперанского Департамента здравоохранения г. Москвы; Балтийский федеральный университет им. Иммануила КантаРоссияG.N. Speransky Children’s Municipal Clinical Hospital № 9; Immanuel Kant Baltic Federal UniversityRussian FederationДетская городская клиническая больница № 9 им. Г.Н. Сперанского Департамента здравоохранения г. МосквыРоссияG.N. Speransky Children’s Municipal Clinical Hospital № 9Russian Federation202112112021185377384Copyright © Юдина Ю.В., Аминова А.И., Продеус А.П., Корсунский А.А., 20212021Юдина Ю.В., Аминова А.И., Продеус А.П., Корсунский А.А.Yudina J.V., Aminova A.I., Prodeus A.P., Korsunskiy A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pedpharma.ru/jour/article/view/2064Обоснование

Обоснование. Атопический дерматит (АтД) в последнее время вызывает все больший научный интерес из-за значительного роста заболевания. Важным фактором риска его возникновения является композиция микробиоты кишечника. Связь данного фактора с развитием АтД у детей требует дальнейшего изучения.

Цель исследования – провести сравнительный анализ микробиоты кишечника детей 1–5 лет с АтД и условно-здоровых детей методом 16S-секвенирования рибосомальной РНК (рРНК) бактериальных генов.

Методы

Методы. Проведено одномоментное исследование. Обследовано 60 детей с установленным диагнозом АтД и 15 условно-здоровых детей в возрасте от 1 года до 5 лет. Микробиоту кишечника исследовали методом секвенирования бактериальных генов 16S рРНК.

Результаты

Результаты. Микробиота кишечника детей с АтД и условно-здоровых детей характеризуется статистически значимыми отличиями. Несмотря на отсутствие существенных различий видового богатства сравниваемых групп, дети с АтД имели повышение в метагеноме бактерий типа Proteobacteria, класса Bacilli и Gammaproteobacteria, семейства бактерий Enterococcaceae и Veillonellaceae, рода бактерий Eggerthella, Dialister и Enterobacter, а также снижение относительного количества бактерий типа Actinobacteria, Bacteroidetes, Verrucomicrobia, отряда бактерий Bacteroidales и Bifidobacteriales, семейства Bifidobacteriaceae, Bacteroidaceae, Erysipelotrichaceae, рода бактерий Lachnoclostridium, Roseburia, Prevotella, Coprococcus, Ruminococcus, Faecalibacterium, Bifidobacterium, Bacteroides, снижение вида бактерий Bifidobacterium longum, Faecalibacterium prausnitzii, Bacteroides fragilis.

Заключение

Заключение. По данным исследования установлено, что микробиота кишечника детей с АтД имеет существенные различия в таксономическом составе в сравнении с микробиотой условно-здоровых детей. Повышение типа Proteobacteria, класса Gammaproteobacteria и Bacilli, семейства бактерий Enterococcaceae и Veillonellaceae, рода бактерий Eggerthella, Dialister и Enterobacter не исключает развития данного заболевания, а снижение таких бактерий, как Verrucomicrobia, Bacteroidales и Bifidobacteriales, – может отягощать проявления атопии. Таким образом, обоснована необходимость дальнейшего изучения микробиоты кишечника у детей с АтД с целью установления взаимосвязи данных бактерий с течением заболевания.

Background

Background. Atopic dermatitis (AD) arouses high research interest these days due to its significant morbidity rate. The most crucial risk factor for its development is the intestinal microbiota composition. The correlation of this factor with the development of AD in children requires further study.

Objective

Objective. The aim of the study is to perform comparative analysis of the intestinal microbiota in 1–5 years old children with AD and conditionally healthy children via 16S-sequencing of ribosomal RNA (rRNA) of bacterial genes.

Methods

Methods. We have conducted cross sectional study. 60 children with diagnosed AD and 15 conditionally healthy children aged from 1 to 5 years were surveyed. Intestinal microbiota was examined via 16S-sequencing of rRNA of bacterial genes.

Results

Results. The intestinal microbiota in children with AD and conditionally healthy children has statistically significant differences. Despite the absence of significant differences in species richness of compared groups, children with AD had the elevation in the metagenome of Proteobacteria; Bacilli and Gammaproteobacteria classes; Enterococcaceae and Veillonellaceae families; Eggerthella, Dialister and Enterobacter genus; as well as the decrease in the relative value of Actinobacteria, Bacteroidetes, Verrucomicrobia; Bacteroidales and Bifidobacteriales orders; Bifidobacteriaceae, Bacteroidaceae, Erysipelotrichaceae families; Lachnoclostridium, Roseburia, Prevotella, Coprococcus, Ruminococcus, Faecalibacterium, Bifidobacterium, Bacteroides genus; decrease of Bifidobacterium longum, Faecalibacterium prausnitzii, Bacteroides fragilis.

Conclusion

Conclusion. It was revealed that the intestinal microbiota of children with AD has significant differences in taxonomic composition with the microbiota of conditionally healthy children. Elevation of Proteobacteria, Bacilli and Gammaproteobacteria classes, Eggerthella, Dialister and Enterobacter genus can be the risk factor for this disease development, whereas decrease of such bacteria as Verrucomicrobia, Bacteroidales and Bifidobacteriales can aggravate atopic symptoms. Thus, the need for further study of intestinal microbiota in children with AD is justified to establish the correlation of these bacteria with the disease course. 

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The authors declare that there are no conflicts of interest present.